专家简介：
廖茂富，南方科技大学生命科学学院讲席教授。1999年毕业于清华大学生物科学与技术系，2006年获美国爱因斯坦医学院博士学位，之后在旧金山加州大学进行博士后工作。2013年，解析了TRPV1的冷冻电镜结构，是第一个通过非晶体学方法产生的近原子分辨率膜蛋白结构。2014年，加入哈佛医学院细胞生物学系建立课题组，研究集中在使用冷冻电镜和生化方法来揭示膜蛋白的结构动态和分子机理，以及基于结构与机制的靶向药物开发，建立了研究跨膜转运蛋白的新范式，以通讯作者身份在Nature、Science和Cell发表论文12篇。2022年，全职回国加入南方科技大学，获聘长江学者讲席教授，现任南科大高分辨生物电镜结构研究院院长和冷冻电镜中心主任。
报告摘要：
ATP-binding cassette (ABC) transporters harness the energy of ATP binding and hydrolysis to translocate a multitude of chemically diverse substrates across cellular membranes. Despite decades of studies and many available structures, the underlying mechanisms of specific and nonspecific ABC transporters are still poorly understood. The ongoing revolution of cryo-EM has enabled novel approaches for obtaining deep insights into these highly dynamic membrane protein machines. Through cryo-EM studies of several bacterial ABC lipid transporters (MsbA, LptB2FG and LolCDE), our group has uncovered how different tasks of substrate translocation are accomplished by unique coupling of three processes: ATP binding/hydrolysis, conformational rearrangement of transporter, and transporter-substrate interaction. Importantly, our research experience serves as a demonstration of how single-particle cryo-EM methodology and the mechanistic study of ABC transporters stimulate each other, thus emphasizing the versatile nature and yet-to-be-realized potential of cryo-EM.
