Many functions of nucleotides and nucleic acids involve their interactions with cellular proteins. In this presentation, I will discuss about our recent efforts toward the development and applications of quantitative proteomic methods for unbiased, proteome-wide discovery of proteins that can recognize unique secondary structures of DNA or duplex DNA carrying a structurally defined DNA modification. I will also discuss our recent development of targeted quantitative proteomic methods for interrogating ATP- and GTP-binding proteins at the entire proteome scale. The application of these methods for uncovering novel targets of clinically used kinase inhibitors and for revealing novel drivers and suppressors for melanoma metastasis will also be presented. Through this presentation, I hope to illustrate that quantitative proteomics constitutes a power tool for discovering novel nucleic acid- and nucleotide-binding proteins and for revealing their functions in cells.
Yinsheng Wang received his Ph. D. degree from Washington University in St. Louis after obtaining his BS and MS degrees from Shandong University and Dalian Institute of Chemical Physics, Chinese Academy of Sciences, respectively. He joined the faculty of the University of California Riverside in 2001, where he is now a Professor and Donald T. Sawyer Endowed Founder's Chair in Chemistry. Yinsheng also serves as the Director for the Environmental Toxicology graduate program at UC Riverside. His current research involves the use of mass spectrometry, along with synthetic organic chemistry and molecular biology, for examining the occurrence and biological consequences of DNA damage, for assessing the biological functions of post-translational modifications of proteins, and for uncovering novel nucleic acid- and nucleotide-binding proteins. Yinsheng has trained or in the process of training of over 70 Ph. D. students and post-doctoral fellows, and he has co-authored more than 230 research articles. Yinsheng was named as a fellow for the American Association for the Advancement of Sciences in 2012, and he was the recipient for the inaugural Chemical Research in Toxicology Young Investigator Award from the Division of Chemical Toxicology of the American Chemical Society (2012), the 2013 Biemann Medal from the American Society for Mass Spectrometry and the 2018 EAS Award for Outstanding Achievements in Mass Spectrometry. He was also named the Yangtze River Scholars Distinguished Professor in 2016. Yinsheng was a standing member for the Cancer Etiology study section in 2011-2015 and for the Environmental Health Sciences study section since 2016. In addition, he was appointed as an Associate Editor for Chemical Research in Toxicology in early 2018.